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MS-MLPA and MSP results: M: methylated, U: unmethylated; fold of Aza induction: + > 3 ×, ++ > 30 ×, +++ > 150 ×, - = no change; n.d.: not determined.

- Annie, AThenta.

The U-turn fold is widespread in RNA structures.

In this model, residues G697, A698, and C699 of the prefolded U-turn of SLV initially target residues C632, U631, and G630 of the unshifted SLI and thereby induce formation of the U-turn fold in the SLI loop.

As shown from the NMR structure and previous models of the SLI/SLV complex, the U-turn fold in SLI brings C629 to stack with G630 in proximity of A701.

Upon formation of the U-turn fold in SLI, other hydrogen-bonding and stacking interactions form at the I/V kissing-loop junction to further stabilize the complex and thereby induce the rearrangement of the C626 G633 base pair and the extrusion of C634 within stem Ib.

Moderate increases in the ras homolog gene family U (Rhou, 4-fold), in fibronection (CTGF, 4-fold), and in prostaglandin-endoperoxide synthase 2 (Ptgs-2, 5.5-fold) were also evident.

Maximum U uptake (3.5-fold) in the roots occurred at 2.5 mmol of CA, while NTA proved to be the weakest for the same purpose.

BSV_X04 does show mispairing at C5 and A34 and breathing at the top loop, but the sequence still retains the bulged U motif and folds into a stable stem loop (delta G = −6.8 kcal/mol).

To be eligible for inclusion in this analysis, subjects were required to have a confirmed diagnosis of pancreatic adenocarcinoma (either locally advanced or metastastic), a baseline serum CA19-9 level greater than 75 U cm−3 (two-fold the upper limit of normal), no prior systemic therapy, and intact renal, hepatic, and haematologic function.

Genes u p-regulated >2.0 fold or down-regulated <0.5 fold by microarray (Table 2) and up-regulated >3.0 by SSH were chosen for analysis.

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