Ms. Boo graduated from Barnard in the late '80s, still typing — for The Columbia Daily Spectator, for which she wrote editorials — and was hired by Jack Shafer, then the editor of the Washington City Paper.
HLA class I typing for the A and B loci was performed using a PCR microtiter plate hybridization method (WAKFlow® HLA typing kit) (Wakunaga Co. Ltd., Hiroshima, Japan), according to the manufacturer's instructions.
Pre-derivation HLA-class I typing for the embryos was carried out using PCR amplification with sequence-specific primer (PCR-SSP) based on the method of Bunce et al (1995).
Typing for the TH and D11S4046 microsatellite markers and the rs11023840 single nucleotide polymorphism (SNP) demonstrated the presence of a de novo maternal duplication in the SRS patient 1 (Fig. 3A and B).
We have developed an assay for typing for the HLA-B*27 in whole blood dried on sample collection cards using pre-dried reagent wells and homogeneous time-resolved fluorescence based PCR approach.
We sought to confirm or extend those findings with application of high resolution HLA typing in a cohort of subjects with PHFM, as high resolution typing may be more useful than low resolution typing for the determination of likely causative genes rather than linked variations [ 10].
