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Highly efficient cell-free enzymatic systems are typically difficult to achieve in traditional immobilized enzyme systems because of the lack of optimal spatial control of enzyme localization, substrate and product diffusion, and enzyme and coenzyme accessibility.
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In cold weather, construction sites typically find it difficult to achieve quality results when preparing concrete structures.
However, considering the dynamic nature of radio channels typically, it is difficult to achieve a given service provisioning working at the IP and lower layers separately as in the classical approach, without a run-time adaptation of the system towards the target quality.
But this balance is difficult to achieve, since, typically, when we drop a pound while dieting, as much as a third of that loss can come from muscle, with the rest composed of fat.
Eco-friendly buildings are typically smaller scale, because human comfort is difficult to achieve in systems dependent on varying climates.
This is because comprehensiveness in such analyses is difficult to achieve, given the typically patchy taxonomic distribution of available data [ 19, 20] that can be analysed in a single matrix.
The convergent mechanisms typically found in standard Evolutionary Algorithms are more difficult to achieve with IES.
This is difficult to achieve using existing technologies, which typically use conventional molecular biology techniques to fuse gfp to the 5' or 3' end of the target gene [14] [16].
It has been also found that it is more difficult to achieve pure conduction welds with small beam diameters, which are typically used in powder bed additive manufacturing, due to high conduction losses and low vaporisation threshold.
It is generally acknowledged that genome-wide associations typically only become replicable in panels of ∼2,000 cases versus controls (Wellcome Trust Case Control Consortium 2007), and of course this sample size is difficult to achieve in studies of the extremely old.
Typically NHEJ is strongly favored in cells and thus integration of desired DNA into a given genome location by HDR is difficult to achieve.
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CEO of Professional Science Editing for Scientists @ prosciediting.com