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We propose that the typical transcript abundance distribution in eukaryotic cells is log-log-normal, consistent with previously published results in which the shape of transcript abundance distribution appears to be log-log-normal [ 9, 12].
Typical transcript half-life times in mammalian cells are in a range from tens of minutes to several hours [ 18], but can of course vary significantly depending on the gene and regulatory influences, with an estimated variation of 200 fold among different genes [ 19].
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The overall decline of MGAT1 expression throughout spermatogenesis reflects a typical somatic transcript dilution effect (compare Figure 1 in Cappallo-Obermann et al., 2013), due to increasing numbers of germ cell-specific transcripts.
Recommended hybridization temperatures for long synthetic oligomer arrays may prove to be closer to 65°C, when only 50% of a typical RNA transcript or 30% of the corresponding cDNA molecule remain inaccessible.
This revealed that EpCAM+ and mammaglobin positive (hMAM+) cells represented a cell population also expressing other typical epithelial transcripts.
Moreover, only four ESTs corresponded to the typical chimeric transcripts observed in teleosts, (utilizing exon Cμ1) and presented the typical VH-D-JH rearrangement.
This contrasts with typical histone transcripts that lack poly-A tails but contain short 3′-stem loop UTRs, which facilitate coordination of histone expression with the cell cycle.
Moreover, this repression was correlated with a global down-regulation of typical corresponding transcripts (APX1, sAPX, DHAR2, and MDAR2), which, conversely, have already been shown to undergo important induction during responses to several environmental abiotic stresses.
Other typical ovarian transcripts were also present in ovary-specific Cluster 1 (see Additional file 3), including the aquaporin 1a homologue, previously detected in T. thynnus ovaries [ 22, 23] and involved in the process of oocyte hydration prior to ovulation, which is necessary for providing buoyancy in pelagic fish eggs [ 64, 71].
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