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With typical expression levels of common fluorescent labels and sensors, the method obtains a correction in the range of <100 ms to several seconds, and is insensitive to sample movement or variations in fluorescence intensity (for example, photobleaching, calcium or voltage transients).
Because each value was median-centered, the standard deviation also represents a surrogate for CV in the population; it is scaled to the typical expression levels.
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Furthermore, with typical transgenic expression levels of 1 4% of total soluble protein (TSP), there are few, if any, host systems that can produce such levels of foreign protein based on weight.
It seems likely that these observations represent typical ASPM localisations and expression levels in 'normal' control cells.
Expression levels of typical pro-inflammatory, anti-inflammatory and chemokine genes were not significantly different with the exception of increased TNFα and Ccr1 mRNA expression in the HuNOS2tg/mNos2-/ mice compared to WT or mNos2-/ mice.
After having maintained EPCs in culture for four days after confluence, we observed a significant increase in p16INK4A expression levels with typical nuclear localization (Fig. 2B, 2C,).
We confirmed that these cells were under ER stress by measuring expression levels of typical ER stress markers, spliced XBP-1 and BiP.
Lack of CYLD expression was reported to lead to constitutive activation of the canonical NF-κB signalling cascase in B-cells as shown by higher expression levels of typical NF-κB target genes (Jin et al, 2007).
Accordingly, we examined mRNA expression levels for typical inflammatory and cytotoxic mediators in the co-culture system, wherein the ratio of B16F1 cells, DCs, and NK cells is 1 30 10.
With data from 26.7 million reads comprising 9.4 × 10 bp from 3 centenarian and 3 control individuals, we discovered a total of 276 known miRNAs and 8 unknown miRNAs ranging several orders of magnitude in expression levels, a typical characteristics of saturated miRNA-sequencing.
A typical trade-off occurred between soluble expression levels and specific activity for all single mutants, but many double mutants decreased both properties more severely suggesting a critical loss of protein stability or native folding.
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