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Typical exposure time was 500 ms.
The typical exposure time is about 1.2h, 1.8h, 0.5h, corresponding to PMMA thickness of 500μ m, 200 μ 20 μ m, respectively.
The SHG images are obtained by scanning the sample with respect to the incoming beam using computer-controlled stepping motors with a minimum step of 100 nm, and recording the SHG signal at each scan step with a typical exposure time is of 20 40 ms per step at a power of 100 150 mW.
The typical exposure time was 15 45 s.
Typical exposure time for each scan on the CCD was 20 s, and spectra reported correspond to an average of 5 20 consecutive scans on the same point.
The typical exposure time for taking diffraction patterns was 1 s for the CCD detector or 3 min for the IP detector for each run.
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Typical exposure times are 6 to 50 msec.
For small proteins like lysozyme collected on F2 station using typical exposure times, 4 mg/ml will usually produce a profile that is dilute enough to avoid interparticle interactions, but strong enough to give a good low-noise Guinier plot with accurate radius of gyration.
Typical exposure times were 30 60 ms for the SGFP and Nomarski images.
Typical exposure times for both excitation wavelengths were 400 ms. A 512×512 pixel back thinned EMCCD camera (C9100, Hamamatsu, Bridgewater, NJ, USA) was used for fluorescence detection.
Typical exposure times were 2×60 s.
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