The expressions of these types of transcripts or genes were confirmed by reverse transcription polymerase chain reaction (RT-PCR).
Besides the transcript variants resulted from alternative splicing, transcriptions using alternative TSSs or PASs generate different types of transcripts with variations in the 5'UTRs or 3'UTRs, which are more likely to be involved in regulation of gene expression compared with other (internal) exons.
For subsequent analyses, we considered two types of transcripts: protein-coding transcripts and long non-coding RNAs (lncRNAs).
This hints at a previously unknown source of the complexity of the brain, given that most genes express five or fewer types of transcripts, and half encode just one protein.
As for BMP-4 expression, a reverse transcription-polymerase chain reaction (RT-PCR) assay with specially designed sets of primers demonstrated that osteoblastic SaOS-2 and Hos cells expressed two types of transcripts comprising one of the 5′-untranslated first exons, whereas MG63 cells displayed only the transcript with the BMP-4 proximal first exon.
The existence of these types of transcripts is supported by and consistent with EST data.
Alternative splicing changes the use of exons, producing multiple types of transcripts from a single gene.
All types of transcripts annotated by Ensembl, as well as ESTs, were included in our analysis.
Surprisingly, we amplified cDNAs for each of the alternative 3'-ends from brain RNA, confirming two types of transcripts are generated from the honeybee neurexin 1 gene (AmNrx1).
