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It can code for all types of transcript based proteins, is easy and cost efficient to produce, has a favorable safety profile and enables induction of combined immune responses.
Both types of transcript produced essentially the same RNAi phenotypes.
Based on the site of breakpoint in t(9 22) (q34 q11), bcr-abl fusion in leukemia patients is associated with different types of transcript proteins.
We computed the 5'ss score distribution to study the relationship between different types of transcript coverage and sequence-complementarity of base pairing to U1 snRNA.
Figure 5B shows clearly that both types of transcript, with and without the BpmI site, are present in nuclear and cytoplasmic compartments of non-DM cell line SBTeloMyoD.
RefGenes therefore allows to select experimental conditions that are similar to that of a specific experiment and to obtain reliable and condition-specific candidates for the normalization of RT-qPCR or other types of transcript quantification data.
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The expressions of these types of transcripts or genes were confirmed by reverse transcription polymerase chain reaction (RT-PCR).
As for BMP-4 expression, a reverse transcription-polymerase chain reaction (RT-PCR) assay with specially designed sets of primers demonstrated that osteoblastic SaOS-2 and Hos cells expressed two types of transcripts comprising one of the 5′-untranslated first exons, whereas MG63 cells displayed only the transcript with the BMP-4 proximal first exon.
The existence of these types of transcripts is supported by and consistent with EST data.
Alternative splicing changes the use of exons, producing multiple types of transcripts from a single gene.
All types of transcripts annotated by Ensembl, as well as ESTs, were included in our analysis.
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CEO of Professional Science Editing for Scientists @ prosciediting.com