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The complete set of (supported or unsupported) non-inferior solutions, consisting of combinations of multi-compartment containers for the disposal of four types of sorted waste in nineteen candidate sites, and population assignments, was generated.
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Two experiments were designed to evaluate the effect of different insemination times (12 and 24 h or 18 and 30 h) and different types of semen (sex-sorted or non-sorted sperm) on embryo production in Nelore (Bos indicus) and Holstein (Bos taurus) superstimulated donors.
Thus, we initially examined the expressions of IL-4 and IL-6 in different populations of sorted cells from wild-type mice.
To assess which types of structural progenitors were enriched within sorted MEC populations, ML, LPC, MB, and BPC were sorted based on EpCAM and CD10 expression and were grown at clonal density on collagen.
While tfFACs represents a new methodology to isolate and characterize similar cellular types from a complex mixture, it does not allow continued growth of sorted cells and thus their lineage specific commitments cannot be readily assessed.
Confocal imaging of sorted mature SP thymocytes showed that both wild-type and mutant Zfp335 localized to the nucleus, forming punctate foci within regions of euchromatin.
To further assess whether the populations of sorted cells provide accurate representation of the targeted prostate cell types, we compared the expression of genes documented in the literature as primarily expressed by luminal, basal, or stromal cells with our sorted luminal, basal, and stromal datasets (Fig. 3).
Quality of sorted cell fractions was verified using RT-QPCR with sets of primers for cell type specific markers as previously described.
To assess the purity of sorted cells, reanalysis of sorted cell populations was performed at various times during sorting.
The purity of sorted cell populations from the adult CNS was confirmed by evaluation of mRNA levels of cell-type-specific genes in individual cell populations.
"It kind of sorted itself out.
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