Among many types of FCs, proton exchange membrane FC (PEMFC) is one of the most promising power sources due to its advantages, such as, low operation temperature, high power density and low emission.
With the recent availability of a wider range of FCs, it is important to know if women with experience in using one type of FC are more proficient in using another type, even if the FC design is quite different.
In fact, the effector functions triggered by IgG antibodies are highly dependent on the type of Fc receptor that is bound; however, many aspects can influence Fc receptor binding by IgG antibodies, including the IgG isotype and the composition of the glycan on the IgG antibody.
Total units were calculated for each type of FC and compared by age group (adult >17 vs. pediatric ≤17 years).
The reactivity of complexes of the human recombinant anti-TPO aAbs and patients' sera with TPO was analysed using cell lines 11A1.6 CD64+, CDw32-L CD32+ and Jurkat CD16+, which express only one type of Fc γR (CD16, CD32 or CD64).
Flow cytometry studies using CD16, CD32 and CD64 monoclonal antibodies showed a sharp reduction on the expression of CD64 both by human monocyte-derived macrophages and THP-1 cells after incubation with LDL, suggesting preferential engagement of this type of Fc receptor [ 26].
These are also common targets for therapeutic mAbs (Reichert, 2011), which have been studied for a much longer period of time than Fc-fusions, although they can actually be considered as a particular type of Fc-fusion construct (Table 1).
