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The uninjured Rag1 −/− mice displayed the similar myofiber size to wild type (Supplementary information, Figure S1A).
In addition, cas002 mutant embryos have significantly fewer EGFP+ cells (marked by eGFP under cmyb promoter) than the wild type (Supplementary information, Figure S6A-S6G).
Truncation of constant regions did not change the specificity of Apt19 as full-length Apt19 showed similar binding profiles towards different cell types (Supplementary information, Figure S1D and S1E).
The difference of the connectivity and directionality of edges around H3K4me3 between the hESC and the CD4+ T-cell network is likely to reflect the diverse regulatory program in the two cell types (Supplementary information, Note S8).
KCNQ2 F137A exhibited similar sensitivity to 10 μM retigabine to wild-type channel (Supplementary information, Figure S2B).
Similar amounts of MuSCs were present in both Rag1 −/− and wild-type muscle (Supplementary information, Figure S1B and S1C).
We used BMDMs from Erk1-knockout and wild-type mice (Supplementary information, Figure S4B) to further test the involvement of Erk1/2.
The injection of mitochondria in Tlr9 −/− mice also showed reduction in pulmonary inflammation compared to wild-type mice (Supplementary information, Figure S8).
For instance, both Kv1.2-2.1 Kv1.2-2.1 KCNQ2 F233W exhibit andlower rate of closure than wild-type channels (Supplementary information, Figure S6C and S6D).
Rag1 −/− mice showed significantly lower number of MuSCs after muscle injury compared to the wild-type mice, while the number of MuSCs in uninjured muscles remained similar in Rag1 −/− and wild-type mice (Supplementary information, Figure S1C).
71, 72 Genes involved in the specification of multiple retinal cell types (see Supplementary Information) are not always included, in a given seed network due to the lack of strong evidence they interact with other essential genes in the seed network specifying a particular cell type.
