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Transverse hippocampal slices (350 µm) were transferred to a custom made interface type recording chamber and incubated for at least 2 hours at 32 degree under constant perfusion with carbogenated artificial cerebrospinal fluid (ACSF containing in mM: 110 NaCl, 5 KCl, 2.5 CaCl2·2H2O, 1.5 MgSO4·7H2O, 1.24 KH2PO4, 10 glucose, 27.4 NaHCO3, pH 7.3).
The slices were then transferred to a gas interface type recording chamber that was perfused with aerated [95% (v/v) O2/5% (v/v) CO2] ACSF (22 24°C), at a rate of 0.5 1 ml/min, by a peristaltic pump-driven or gravity-fed bath-perfusion system (Choi et al., 2008).
Employers need to tie banning any type recording to directly to a precise business need, such as patient privacy.
The primary outcome measure was the proportion of children cured of bilateral otitis media with effusion, assessed with tympanometric criteria at one month (proportions of children with at least one ear with an A or C1 type recording), because children with bilateral otitis media with effusion are deemed to be at greater risk of disability than those with good hearing in one ear.
Similar(56)
In each quadrant, three teeth were identified and the restoration type recorded.
In what is possibly the largest event of its type recorded this catastrophic loss of life might have been caused by a lightning strike.
The former type reflected derivation of major ions largely from water rock interactions, while the latter type recorded varying degrees of anthropogenic contamination.
Nevertheless, we were at least able to detect the application types using unknown device type records from the dictionary.
The output type records whether a research output is a journal article, a book, a building, etc.
Superficial spreading melanoma was the most common histological type recorded, followed by nodular melanoma.
Slices (300-µm thick) were cut on a Leica VT1200S and incubated in cutting solution in a holding chamber at 32°C for approximately 30 min followed by continued incubation at room temperature prior to electrophysiological recording, at which point slices were transferred to a submersion-type recording chamber attached to the microscope stage.
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