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The single mutations induced a leukemia similar to wild type when transplanted into mice, however the double mutant induced an undetermined disease with recipients surviving twice as long as wild type recipient mice [25].
To do this, IKMV donor mammary tissue was transplanted into the cleared fat pad of 3 week old FVB wild type recipient females.
With regard to the current studies, IKMV donor mammary tissue from 3 4 week old donors was transplanted into the cleared fat pad of the left inguinal mammary gland of 3 week old FVB wild type recipient females.
Consistently, the endogenous Cfl1-mCherry in the wild type recipient strain is induced by released signals from the donor strain in a distance-dependent manner, implicating extracellular Cfl1 as a paracrine signal.
Logistic regression analysis was used to determine significant associations between studied variables and 6-month presence of anti-HLA antibodies, using a multivariable model that included variables presenting P ≤ 0.1 in univariable analysis (ATG use, time on dialysis, kidney-pancreas graft, acute rejection type, recipient age, donor age, and ABDR mismatches) (data not shown).
***p<0.001 DOI: http://dx.doi.org/10.7554/eLife.07539.006 To rule out non-cell autonomous effects of Ddb1 deletion (i.e. effects in HSC niches), we transplanted cells from non-polyI C inon-polyI C1 f/f::Mx1Cre +minjected lethally irraDdb1ed wild type recipient mice.
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To this end isolated cells were stained with fluorescent dyes (CellTracerCFSE, CellTrackerOrange or CellTracerViolet), adoptively transferred into wild type recipients and then tested by various assays.
The effects of either GAG or chemokine receptor deficiency on vascular neointimal hyperplasia (plaque) growth, was examined in mouse aortic allograft transplant models with donor aortic genetic deficiency of Ndst1 or Ccr2 (Table 1, n = 79) and wild type recipients.
FACS data for wild type recipients are very similar.
FACS data for wild type recipients are very similar (not shown for space considerations).
Wild type bone marrow was transplanted to Shb +/− recipients and Shb +/− bone marrow was transplanted to wild type recipients.
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