Preparation of the nursery will depend on the type of seedling to be produced (bare root or polybag) and it is also necessary to make allowance for the shape of the seednut (angular or spherical).
We found in our previous observation that the seedlings from brown seeds of S. aralocaspica emerged earlier and with higher uniformity than those of black seeds; however, the difference between two types of seedlings became indistinguishable in 2 3 weeks.
It suggests that the difference between two types of seedlings in the early stage in S. aralocaspica arose because of different rates of emergence time rather than for other reasons.
We sequenced small RNAs from five types of tissue: seedlings, roots, petioles, leaves, and flowers.
Three SSRs were mapped close to this locus on LG5: CH03a09, Hi04d02 and CH05f06; however, the frequency of 'Type 1' seedlings bringing the incompatible allele of the SSR CH03a09 (115 bp, denoted as l) was only 48.8%, and hence, this marker might not belong to the region linked to 'Type 1' lethality.
For the second class of HN, the highest frequencies of 'Type 2' seedlings bringing the incompatible allele were detected for markers CN493139, LETss527788384 and CN444636, mapping to LG2 of PEAR3 (Table 3), and the segregation distortion was stronger in the region within those markers.
The frequency of 'Type 1' seedlings carrying the incompatible allele inherited from LG1 of 'Moonglow' is higher for SSR CHVf1 than for the markers flanking it (Table 3), indicating that the lethal gene is closely linked to this SSR and located between markers MDP0000160413_LG1b and MDP0000251943_LG1b, which spanned a region of 8 cM.
These results suggest the differential response of rice GST genes with respect to age of seedlings, tissue-type and/or cytokinin type.
This reduction likely results from the mixed genotypes of the phenotypically wild type sibling seedlings consisting of FLN/FLN and FLN/fln1-1 genotypes.
The most extreme situation was observed for markers LETss527789863 from LG5 of PEAR3 and MDP0000160413_LG1b from LG1 of 'Moonglow', for which 44 out of 54 'Type 1' seedlings (85.5%) carried both the b alleles of the first marker and the n allele of the second marker, while only eight out of 166 'Type 2' + 'Type 3' seedlings (4.8%) carried these alleles in combination (Table 2).
