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71% of the transfected cells (N = 236) displayed long filopodia, but the remainder did not show this type of protrusion.
Three main factors have been proposed to control the type of protrusion formed by a cell: actomyosin contractility, actin polymerization and substrate adhesiveness [ 48].
We then review the processes involved in determining the type of protrusion formed by migrating cells, in particular in vivo, in the context of embryonic development.
Our knowledge of the mechanics and regulation of bleb formation has been considerably broadened in the past five years, and the factors controlling the type of protrusion formed by a migrating cell are now better understood.
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Modulation of these parameters, individually or in combination, can dictate the specific type of protrusions migrating cells form.
Many cells such as Dictyostelium are able to apply both crawling and blebbing motion, whereas others exclusively use one type of protrusions [ 342, 358, 359].
However, two types of protrusion, bright and dark, are observed and they are ordered in a c (4 × 8) network.
As shown in movie S1 and S2, the leading edge hypoblast cells of a StdMO morphant formed different types of protrusion but mainly the long and thick lamellapodia at the lateral side where cells converge actively toward the dorsal side (arrow, Fig. 8A).
The development of electron beam Surfi-Sculpt enables many types of protrusions in a wide range of metallic materials to be tailored.
Melanoblasts migrate individually within the developing mouse embryo epidermis using two types of protrusions: short stubs and long pseudopods (Li et al., 2011).
These types of protrusions are characteristic of mobile B lymphocytes (Anderson et al., 1979) and not a defect caused by overexpression.
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