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Depending on the type of mutant, these complexes may retain a degree of COX-2 activating capacity.
But one type of mutant fly seemed less able to connect the two stimuli even for a few minutes; they learned to avoid the odor half as often as normal flies.
This type of mutant designated SsrAresume was not tested before in vivo for essentiality in another organism.
In contrast, the bacteria in the symbiotic cells of the second type of mutant nodule were not elongated (Figure 1C).
The third type of mutant was identical to its parent in all respects (a 'null mutant'), including the ability to use NO3, NO2 and NH4 (Fig. 1B).
It is remarkable that, in this type of mutant, the nodule organogenesis program, which is costly, is maintained rather than aborted through arrest of meristematic activity and prevention of further cell infection.
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The NCBI collection of avian sequences had 2 out of 5991 (0.03%) mutants with more than a single mutation, while in the Ottenby collection the same type of mutants was found in 4 out of 230 (1.74%) isolates.
The authors note, however, that the high frequency of large-effect deleterious mutations that they observed may be an artifact of the particular type of mutants generated.
Also, some new model predictions are made for an in vitro type of mutants, in which the formation of specific dimers is artificially repressed.
Despite thorough screenings they could not obtain the desired type of mutants, which in their view suggested that the photoreceptor could be essential.
However, long and accurate PCR by a single-type of mutant DNA polymerase was very difficult.
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