From left to right: Kitaake wild type (Kit), transgenic line (XA21, 23A-1-14) carrying XA21 driven from its native promoter, and transgenic Kitaake lines carrying XB21ox construct (XB21ox/Kit).
From left to right: Transgenic lines carrying XB21ox construct (XB21ox/XA21), transgenic line (XA21, 23A-1-14) carrying XA21 driven from its native promoter, and Kitaake wild type (Kit).
b Lesion lengths of Xoo inoculated plants of transgenic lines carrying XB21ox construct (XB21ox/XA21), transgenic line (XA21) carrying XA21 driven from its native promoter, and Kitaake wild type (Kit).
b Lesion lengths of Xoo inoculated plants of Kitaake wild type (Kit), transgenic line (XA21) carrying XA21 driven from its native promoter, and transgenic Kitaake lines carrying XB21ox construct (XB21ox/Kit).
In figure 2A, we show two typical leaves from each of the inoculated rice plants: wild type Kit, Nat XA21 control, and BiP3 ox/Nat XA21 at 14 days after Xoo strain PXO99Az inoculation.
The plasma level of SNE was measured on the first, third and fifth day of hospitalization, using the ELISA technique with an immunoenzymatic method, sandwich type (kit CanAg Diagnostics), in a Dias Orin automate (Eti-max 3000).
We genotyped HLA-B using the Luminex assay system and HLA typing kits (WAKFlow HLA Typing kits, Wakunaga, Osaka, Japan or LABType SSO, One Lambda, Canoga Park, CA, USA).
We genotyped HLA-A, HLA-C, HLA-B, HLA-DRB1, HLA-DQB1, and HLA-DPB1 using the Luminex assay system and HLA typing kits (WAKFlow HLA Typing kits, Wakunaga, Osaka, Japan or LABType SSO, One Lambda, Canoga Park, CA).
B-cell lines were HLA-DR-typed using commercial, polymerase chain reaction-based, low-medium-resolution, DRB1 typing kits (Dynal Biotech, now part of Invitrogen Corporation, Carlsbad, CA, USA) followed by high-resolution, DRB1 allele-specific typing when indicated.
Commercial HLA-Type kits were used for determination of the HLA-DRB1 and HLA-DQB1 allele frequencies.
Commercial HLA-Type kits from BAG (Lich, Germany) company were used for determination of the HLA-A and HLA-B allele frequencies in genomic DNA, based on polymerase chain reaction with sequence specific primer (PCR-SSP) assay.
