Briefly, subchondral bone specimens were digested by sequential collagenase type I digestion, followed by cell culture in BGJb medium containing 20% FBS.
To confirm that the isolated plasmid was the Dutch type plasmid, digestion with BglI was performed and the obtained digestion pattern was similar to the previously observed pattern for a Dutch type plasmid [20].
So, we decided to use only this type of digestion, which fully mimics the human gastro-intestinal digestion, for the following more complex and time-demanding experiments of this study.
Rations are influenced by factors such as the species, type of digestion, chemical composition, and presence of secondary compounds (Mackie 2002; Michelland et al. 2012).
Analysis of variance showed that all main effects (microwave temperature, sludge type and digestion temperature) and two-way interactions have significant (P-value < 0.05) effects on cumulative methane production relative to control (not pretreated) digesters (CMPr) at elevated microwave temperatures (150 175 °C).
Fortunately, protease type XIII digestion successfully generated 11 overlapping peptides covering region 221 241.
Synovial membranes were removed from the right hind-knee joints of experimental rats, and cells were isolated by collagenase type IV digestion, as described [ 29].
Finally, for 13% (CI95% = 0-27) of children, LIRs were useful for one any type of digestion but not for the other.
The wheat and wild type pea digestion plots shown in Figs. 2 and 3 are taken from Butterworth et al. (2012), with the addition of the LOS plot for 24 h retrograded wheat and wild type pea starch.
HUVECs were isolated from sterile, freshly obtained umbilical cords at the time of a normal delivery by using 15 mg/mL collagenase type I digestion as previously described [ 20, 21].
