Fig. 3 Proteins up- or down-regulated twofold and significantly varying with water deficit (WD), Heat, and combined water deficit + heat (WD + H) stress compared with Control (at 5%) were excised from 2D gels, sequenced using MS MALDI-TOF, and identified by database searches using peptide mass fingerprinting data.
Fig. 4 Proteins up- or down-regulated twofold and significantly varying with water deficit (WD), Heat, and combined water deficit + heat (WD + H) stress compared with Control (at 5%) were excised from 2D gels, sequenced using MS MALDI-TOF, but not identified from the available databases using peptide mass fingerprinting data.
qPCR experiments confirmed that the expression of COL7A1 was variable in different clones (Fig 3B), with levels of mRNAs varying from twofold to fiftyfold (clone 3 and clone 58, respectively) compared to uninfected RDEB keratinocytes.
An arbitrary twofold cutoff was implemented to identify cell cycle varying proteins.
This private jail, and the policing system that governs it, is replicated to varying degrees in other department stores across the nation with a twofold purpose: to stop shoplifting and to recoup some of the billions of dollars lost to theft every year.
However, despite almost twofold differences in exon size in various species, the total GC content in exons is conserved, varying from 41.4 to 42.6% (Table 5).
In vitro- in vivo extrapolation methods (hepatocytes or microsomes) for hepatic clearance predictions have varying success rates reported in the literature, ranging from 20 90% of compounds predicted within twofold.
Three replicate abundance ratios under control, water deficit, heat, and combined stress were determined and spots varying significantly both at 5% (P < 0.05) and with approximately greater than or equal to twofold changes were considered for further analysis.
With widely varying results.
