To be classified as positive, the value of a given recipient's serum sample has to exceed twofold the value of the negative control serum.
Wells were counted as positive if they contained at least 50 SFCs/106 PBMCs and exhibited at least twofold the mean value of the background (per million PBMC).
The value of the article is twofold: it briefly reviews the discoveries of hominins in Africa and interviews a number of researchers who have studied these fossils, as well as others.
A serum sample was considered potentially positive for anti-belimumab if the mean A450 value of the postdose sample was at least twofold greater than the mean A450 value of the predose sample.
The standard curve was produced by measuring the crossing point of each standard value (twofold serially diluted cDNAs from the Human Universal Reference Total RNAs) and plotting them against the logarithmic value of the concentration.
C4S had the most prominent effect on the degradation of types I and II collagens by cathepsin K [ 41], whereas its effect on the hydrolysis of a synthetic substrate was virtually identical to that of C6S and DS and resulted in a twofold increase in the values of the specificity constant (kcat/ K m ).
These are twofold: first, the value of RLI must be greater than 1 and, second, the partial χ value must account for a significant proportion (say 10% or more) of the total.
These perturbations were chosen to be uniformly distributed with twofold range of the original value (see Supplementary Data online, paragraph E.2) and thereby lower than the reported accuracy of the in silico prediction methods of a factor of 3. We compared these two sources of variability based on the generic PBPK model, using lidocaine (400 mg via 60 min i.v. infusion) for illustrative purpose.
Comparing our results with the other available pediatric model for the oral application, Parrot et al. reported in a model for the oral oseltamivir and its metabolite a predicted AUC in infants that was within a twofold range of the observed value (19), which resembles our finding using both software for the same age group.
Alternatively, the LD formation was monitored by flow cytometry, with ML-infected macrophages showing twofold higher values of Mean Fluorescence Intensity (MIF) with Nile red probe in comparison to uninfected cells (Fig. 1F).
