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The dashed lines indicate twofold up- or down-regulation of miR expression levels after treatment.
Dark gene, twofold down; light green, eightfold down; orange, twofold up; red, eightfold up.
Dark green, twofold down; light green, eightfold down; orange, twofold up; red, eightfold up.
In general, the fold changes are very small, with the large majority being less than twofold up or down.
We filtered out genes that did not change at least twofold (up or down) at any time point, and we used the default values for all other parameters.
Figure 5a outlines how each comparison was performed and the number of genes identified as at least twofold up and downregulated in each pairwise comparison.
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The microarray analysis of PC12 cells cultured for 4 days under simulated micro-g under standardized oxygen environment conditions revealed more than 100 genes whose expression levels were changed at least twofold (up-regulation of 65 genes and down-regulation of 39 genes) compared with those from cells in the unit gravity (unit-g) control.
Over twofold up-regulation in FRs of contigs representing C4H and 4CL, as well as of contigs of coniferyl-alcohol glucosyltransferase was apparent.
At ATRA concentrations of 10−7 m, a twofold up-regulation of COL 7A1 gene expression was demonstrated with no further change in expression at higher concentrations (Table 1).
In this context, we observed an approximately twofold up-regulation of caveolin-1 expression in OL treated with NGF for 48 h compared to OL treated with NGF for 4 and 24 h or untreated OL (Fig. 1).
Moreover, the twofold up-regulation of PIN5 and PIN7 detected in OE1 plants compared with WT and KO plants also reflected the different intracellular auxin concentrations among the WT, KO and OE1 lines.
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