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6 Since the Dutch COTiD programme demonstrated such highly positive effects, we judged it as appropriate to conduct not an identical replication, but a twofold transfer from the source to the target country and from a mono-centre RCT design with high expertise of interventionists to a pragmatic multicentre RCT design in routine care.
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However, even after multiple hypothesis testing, 99 unique genes were deregulated more than twofold in U937T_pUHD10S cells transferred to tetracycline free media.
Suggested mechanisms of this transfer are probably twofold: 1) enzyme-mediated electron transfer through the redox centers of plasma membrane oxidoreductases, and 2) transmembrane metabolite shuttling/cycling (particularly ascorbate/dehydroascorbate cycling by plasma membrane conduits and/or exocytosis) [ 4].
One-hundred-microliters of 2× concentration of drug were added to columns 1 and 2. The antibiotics were serially diluted twofold in consecutive columns by transferring 100 μL, except for column 10, where 100 μL of excess medium was discarded.
For both sensors sensitivity was rather modest, with 60% quenching of the fluorescence signal observed for the quenching probe and a twofold increase in the energy-transfer signal with the same concentration of thrombin as for the FRET-based aptamer.
The resulting culture of 100 µL was transferred and mixed with twofold serial dilutions of compound or ciprofloxacin.
Mass transfer results provide evidence of a twofold to threefold increase in membrane utilization over other designs in the existing literature.
Our results indicate that intensive tillage has a twofold influence: aggregate redistribution due to the soil transfer process and the mechanical breakage of macroaggregates.
The transfer of solar heat is a twofold process wherein the energy from the collector is transferred first to an intermediate energy storage buffer and the energy is subsequently transferred from the buffer to the cooking load.
This twofold objective is achieved by providing means-tested transfers to low-income households with eligibility conditional on some employment requirement.
Results indicate that considerable heat transfer enhancement is possible, even achieving a twofold increase in the case of a 10% nanoparticle volume fraction nanofluid.
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