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Shorter exposure to dsRNA allowed development to the twofold stage and the few embryos that hatched were abnormal.
Injection of eps-8 dsRNA into the syncytial gonads of wild type N2 hermaphrodites caused 100% lethality of F1 progeny laid between 5 and 20 h post injection (n = 569 progeny of 14 parents); of these progeny, 65.4% arrested at the twofold stage of elongation and displayed detachment of body muscles.
mec-8; sqt-3 embryo keyhole depth was measured by growing embryos at 25°C starting at the twofold stage and quantified every 60 min for 5 hr.
For these experiments, rare mec-8 ; sqt-3 (ts ) mutants that exhibited a keyhole at the twofold stage (∼5%) were analyzed for reasons of experimental convenience.
(B ) Detachment of the pharynx from the epidermis after the twofold stage in mec-8 ; pha-1 (e2123 ); sym-3 mutants leads to loss of the anterior ingression by the threefold embryonic stage and suppression of Pin in L1 larvae.
We also observed that mec-8; sym-4; let-502 RNAi) let-502 RNAi longembryosransitookom the comma stage to the twofolongerge toan transittrol RNAi-treated strain.
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Importantly, the tagged transgene amplified to normal levels and at the same developmental times as the endogenous DAFC-62D, as evidenced by twofold amplification in stage 10B egg chambers and 3- to 4-fold amplification in stage 13 egg chambers.
The authors of the deep sequencing analysis identified as many as 71 snoRNAs up-regulated (more than twofold) in higher stage PCa, but they subsequently focused only on the prognostic role of miRNA signatures.
Especially, the number of stage-specific transcripts decreased more than twofold in the tetrad stage of mutant anthers (10,022 and 4,627 stage-specific transcripts detected in the tetrad stage of WT and mutant anthers, Figure 3b and c).
In 35S::Clineslines, WOX4 expression was increased approximately twofold at all stages of stem development relative to wild type (Fig. 4A).
Moreover, several studies report that there is also a relationship between the severity of AKI and the increased risk of chronic damage with a twofold increase in end-stage CRI requiring dialysis at 10 years [223].
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