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For ChIP-seq analysis the following statistical parameters were used: MACS2 peak calling of MYC ChIP-seq (p-value < 1×10−6); SICER V1.1 peak calling of histone modification (FDR < 0.01); HOMER "getDifferentialPeaks" (cut-offs = twofold change and p-value < 1×10−4).
In flies and worms, epigenetic modifications seem to be less extensive, possibly because dosage compensation here involves fine-tuning of expression levels to a twofold change instead of global silencing, therefore, requiring different mechanisms and different chromatin modifications.
The twofold behavior is actually either retrieval or modification like.
This modification produced on average a twofold reduction in the growth inhibition endpoint (the IC50), suggesting a greater apoptotic response.
The purpose is twofold.
The explanation is twofold.
The reason is twofold.
Their role is twofold.
The mission was twofold.
The worry is twofold.
The task is twofold.
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