Sentence examples for twofold concentrated from inspiring English sources
For stability under various salinities, equal volume of twofold concentrated surfactant and NaCl solutions were mixed to have the final concentration of RL7-11 at 0.12 g/l and NaCl at 0, 4, 8, and 12% in each sample prior to surfactant activity analyses.
Bound material was eluted by incubating in twofold concentrated gel sample buffer for 10 min at 95°C.
Supernatants were diluted 1∶2 into 100 mM sodium citrate pH 6.7, 8% Triton X-100, 20 mM MgCl2, 2 mM DTT, and twofold concentrated protease inhibitors.
Supernatants of UTC buffer extraction containing approximately 2000 cells/μl were mixed 1 1 with twofold concentrated sample buffer (100 mM Tris-Cl pH 6.8, 4% SDS, 20% Glycerin, 3% DTT, 0.05% Bromphenolblue).
Nevertheless, there was again strong evidence of non-linearity and any association appeared to be mainly driven by a twofold increase in risk concentrated only at the quartile of participants who experience the highest levels of exposure.
The effect appeared to be mainly driven by a twofold increase in risk concentrated among those participants who experience the highest levels of emissions, with no much evidence of risk at intermediate levels of exposure or longer distances.
PBMCs were isolated from the remaining EDTA peripheral blood diluted twofold with two-times concentrated RPMI 1640 medium (Gibco, USA) using 50% (v/v) Percoll (Sigma Aldrich) gradient with centrifugation at 400 × g for 30 min. Collected PBMCs were washed twice with RMPI 1640 and red blood cells were then lysed in NH4Cl/Tris buffer for 10 min on ice.
The sample was then concentrated approximately twofold using Amicon Ultra-14 (MWCO = 10 kDa) and subjected to gel filtration fractionation on a Superdex 75 16/60 column (GE Healthcare Life Sciences, Pittsburgh, PA) at 1 ml/min flow rate in buffer A4 (25 mM HEPES/KOH pH 7.5, 200 mM KGlu, 1 mM MgSO4, 1 mM DTT, 100 µM Mg-ATP and 5% glycerol).
For the time-dependent NMR studies of the dimerization of unlabeled p66 with [CH3-Ile]p66∆PL, we mixed the labeled p66∆PL with a twofold excess of unlabeled p66, concentrated the sample, and exchanged it into the NMR buffer: 25 mM Tris-HCl-d11, pD 7.5, 100 mM KCl, and 0.02% NaN3, with Amicon Ultra Centrifual Filters (30 Kda cut-off).
The benefit of bone marrow separation is twofold: Red blood cells are removed since they can interfere with bone formation and healing [6], and concentrated osteoprogenitor cells increase the osteogenic stimulus.
