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Protein spots representing greater than twofold alteration in expression were picked using an Ettan Spot Picker (GE Healthcare Biosciences).
Compared with fish injected with GFP mRNA alone, there was a greater than twofold alteration in the expression of 480 genes, with roughly an equal number of transcripts up and downregulated (Fig. 5A; supplementary material Tables S1, S2).
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More than twofold alterations of the mRNA and protein amount in the tumor samples when compared to the normal ones were taken as a meaningful difference.
Consequently, operational restructuring is twofold through CAPEX alterations (Eichner 2010).
Genes with significant alterations (twofold or more) in expression level are listed in Table 3.
Statistical analysis of data obtained from the normalization and screening procedures was performed to select probes with at least a twofold, statistically significant expression alteration in type 1 or type 2 diabetic animals compared to Wistar controls using Welch's t-test supplemented with the Benjamini-Hochberg multiple correction test with a p = 0.05 cutoff.
The primary FDR-controlled alteration was an approximately twofold increase in ribosome occupancy for a small group of seed storage protein mRNAs.
The importance of these structure alterations was confirmed by the twofold growth of the quantum yield of anthracene photo-oxidation catalyzed by tetraphenylporphyrin (TPP) immobilized on MF-4SK membranes treated with ethylene glycol.
The purpose is twofold.
The explanation is twofold.
The reason is twofold.
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