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In total we tested for twelve two-tailed experimental hypotheses.
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As expected for a continuous protein-coding region, the relative evolutionary rate of 1st and 2nd codon positions was significantly lower than that of the 3rd codon position (two-tailed T-test p < 0.01).
Box limits are the 25th and 75th percentiles, while the whiskers extend to the 2nd and 98th percentiles to delimit a two-tailed 4 % threshold for significance.
We compared samples individually for significant genes i.e. gene ratios, among experimental setups, using a two-tailed z-test, which we refer to as "inter-experimental".
Non parametric Mann-Whitney two-tailed test was used to compare experimental groups and a p value <0.05 was considered as statistically significant.
To assess differences for the theoretic ED30 and experimental ED30, the two-tailed Student t test was used.
To evaluate the significance of differences between control and experimental groups the two-tailed Student's t test was used.
For pairwise comparisons of endpoint means of experimental and control groups, two-tailed t tests were conducted using Prism (GraphPad).
Statistical significance was assessed using an unpaired two-tailed Student's t-test, comparing the experimental data with the respective controls.
We used two-tailed, unpaired t-tests when comparing two experimental groups.
For baseline and experimental measurements between groups, two-tailed Student's t-test was used.
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