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In this line of thought, an easy and efficient two-step validation study has been designed and conducted in our laboratory.
Thus, a two-step validation was carried for all PASS2 superfamilies.
A two-step validation procedure suggests that positive selection was robustly identified in our genomic survey.
A two-step validation strategy was used for both outcomes: DR and DMP (including DMO).
In order to support the results obtained with our microarray analysis, we performed a two-step validation process.
Our two-step validation study addresses the internal coherence (ICS) scale, which is based on expert rating, using specific items for oncological patients, with respect to its reliability, validity and sensitivity to chemotherapy.
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They also mention that they have used two-step validations.
A two-step content validation study was conducted.
Moreover the consistency of the genotyping method and the variant validation criteria was confirmed by the high repeatability (0.94) of duplicates, and by the substantial increase in the repeatability measures between the 3rd and 5th step of variant validation procedure.
For a duplicate each measurement was calculated four times - after the 3rd, 4th and 5th steps of the variant validation procedure, and following supertype classification.
For each duplicate we calculated the agreement between the genotypes after the 3rd, 4th and 5th step of the variant validation procedure, to verify the efficiency of the method (see Table 3).
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