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This notion is supported by a two-step protocol.
Using a simplified two-step protocol, we differentiated these iPSCs into cortical glutamatergic neurons with minimal well-to-well variability.
We developed a scalable two-step protocol for linkage of scFv to preformed and drug-loaded liposomes.
In this work, monodisperse PdCu@PtCu NCs with various shell thicknesses and compositions have been synthesized through a two-step protocol.
In this work, the development of a simple two-step protocol to prepare surfaces able to prevent the bacterial growth was successfully achieved.
Herein, PdAu bimetallic nanoparticles (NPs) with core-shell morphology (PdCore AuShell) were fabricated on the surface of reduced graphene oxide (RGO) support by a facile two-step protocol.
The RT-PCR was performed according to a two-step protocol.
TST was considered positive if induration was ≥10mm (after the two-step protocol).
A two-step protocol was conducted with reverse transcription with gene-specific primers, followed by real-time qPCR with designed TaqMan® major groove binder probes.
Filter values were optimized for best performance in SVM-based classifiers (produced by LIBSVM, linear kernel) according to ten-fold cross validation by a two-step protocol.
For the two-step protocol, 2 separate reverse transcriptions were performed, one for the 5' parts and one for the 3' parts of the segments.
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