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The helices formed as expected, including two wobble GU and Watson Crick A15-U27 and C16-G25 pairs.
Due to the permissive nature of the gRNA-mRNA interaction, the two gRNAs impart identical editing information, although the 8-bp anchor region that provides the initial interaction between the partially edited mRNA and the gRNA in CL Brener is less stable due to the presence of two wobble pairs.
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The other (Lancaster) knows coaching a team rarely means treading a direct line and for every step forward there can be two wobbles back.
A rackety bee wobbles over a flower that is also wobbling in the breeze, and by some complex agreement the two wobbles cancel each other out and the bee is able to land.
The combination of these two wobbles causes the poles to trace spiral paths out of, around, and eventually back into their mean positions over a period of about 6.5 years.
One wobble and your day was wasted.
For these three wobble codon outliers, we see a dramatic increase in short footprints, representing post-decoding stages of translation.
Based on these results, the oligonucleotides for the microarray were designed in such a way that no more than four wobble nucleotides or inosines were introduced as close to the 5′-terminus as possible.
However, we cannot exclude that the use of the stem-loop DNA adapter with seven wobble bases did not hybridize well in such stem-loop regions and therefore did not reach a perfect resolution of 3′-transcript ends.
It's satisfying to see the classic three wobbles before it clicks shut, though it could use some added complexity.
For 12 years, Karen Matthews, mother of seven, wobbled precariously along the line separating adequate parenting and child neglect.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com