After two or three weeks, surviving cell colonies were picked up using cloning rings.
To silence src, cells were infected with the viral particles (1∶200 dilution) in the presence of polybrene (8 µg/ml), and selection was performed with puromycin (Invivogen) for two weeks, whereupon surviving cells were pooled.
"I noticed Longlong's feet swelling at the end of December, just two weeks after surviving [the typhoon]," Maricel says.
After two weeks, return surviving plants to normal growing conditions.
An ambitious manager logs 80-hour work weeks, surviving on five or six hours of sleep a night and eight cups of coffee (the world's second-most widely sold commodity, after oil) a day.
Firtha, who took the fight at two weeks' notice, survived an early onslaught from Fury but recovered to land a fine right hand, which rocked his 6ft 9in opponent.
Performance of lamG262/+ and control flies declined similarly, while homozygotes did not show any decrease beyond their initial low levels over the two weeks they survived, probably because they climbed too little to show any further decrement (Fig. 3B).
And finally, what about all the asshole women who act like not waxing is the same as not brushing your teeth for three weeks, or surviving on a diet of bar nuts and Yop?
ZAMALKA, Syria, Oct 4 (Reuters) - Sixteen-year-old Mohammad al Zeibaa lost his entire family in the sarin gas attack east of Damascus six weeks ago, surviving the world's deadliest chemical weapons strike in a quarter century only because he was out working a hospital night shift.
