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Molecular marker and linkage analyses were carried out according to Cabezas et al. 2006 [ 36] using a two way pseudo-testcross strategy [ 39], and the Joinmap 3.0 software [ 20].
To identify homogeneous subtypes, a two way clustering of the aberration profiles across samples and pathways was done using hierarchical clustering with the same distance metric and linkage method.
In this way, linkage groups were created between markers that were linked with a two-point LOD score > 5, had a minimum of 0.4 times the average number of meioses, shared linkages with no more than six other groups and had a minimum linkage ratio (i.e. the proportion of two point linkages for a given marker to other markers in the same linkage group) of 0.5.
Linkage with large domestic firms is established through two ways: one is through 'independent software vendor' (ISV) programmes, and the other is through the establishment of an India Development Center IDCC).
We probed the linkage between 'substrate scarcity' and polymerase activity in two ways.
Independent linkage maps were constructed for each parent using the two way-pseudo testcross mapping strategy [ 63] by using JoinMap 2.0 software [ 64].
One fish, two ways.
This works two ways.
In two ways, yet.
Two ways, I think.
In two ways.
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