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Proteins in the supernatants were precipitated with two volume of ice-cold acetone, and then the acetone precipitation and cell pellets were subjected to SDS-PAGE (12% (w/v) acrylamide) and western blotting analysis.
Two volume of protein samples were mixed with one volume of 3× Laemmli buffer and boiled for five minutes.
Two volume of ethanol and one-tenth volume of sodium acetate (3 M, pH 5.2) were added and mixed thoroughly.
Similar(56)
Two volumes of Swinburne were stuck together.
He also published two volumes of autobiography.
Also: The first two volumes of Philip Roth's collected works.
King wrote two volumes of autobiography and books on sailing.
I possess only two volumes of this kind.
She was one of the last firsthand chroniclers of Bloomsbury, publishing two volumes of autobiography and six volumes of her diaries, beginning when she was 78.
Two volumes of his letters were published in 1922 and 1924.
She also wrote two volumes of memoirs (1926).
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