In particular, such a proposal can be performed by two types of templates: 1) GENE- inhibited -GENE- expression ; 2) mutant -GENE- inhibited -GENE- expression.
The alternative strategy that we developed makes use of primer sets that do not span introns and therefore amplify genomic DNA sequences that have the same length and composition as their corresponding cDNA's, eliminating any possible difference in PCR efficiency for the two types of templates [[ 17- 19]; C. Hartshorn, A. Anshelevich and L. J. Wangh, in preparation].
The three types of template were analyzed separately by combined bisulfite restriction analysis (COBRA).
To investigate the crystallization conditions of lysozyme, ten types of template MMVs were used to generate 2 (=1024) different conditions.
Three types of templates with a diameter of approximately 10×8 cm were used in the temporoparietal area of plastic skull models which were scanned by helical CT.
To do this, we generated several different head models for four adult participants: first realistic, subject-specific models constructed from subject MR head images, and then best-fitting spherical and four types of template-derived head models.
We compared the consistency of brain morphological features between the original MR images of children participants and the transformed images after the registration to these four types of templates: (1) nationality-appropriate child template; (2) nationality-inappropriate child template; (3) Chinese56 adult template; (4) US20 24 adult template.
For PacBio RS sequencing, two types of SMRTbell DNA template libraries were created with 1- and 10-kb sheared genomic DNA, and prepared using the standard PacBio RS sample preparation methods with C2 chemistry specific to each insert size.
That is, there are different templates for making these two types of points and these templates were transmitted to members of the social group in which each is made (cladistical approaches have similarly been applied to identifying material cultural lineages, e.g., O'Brien and Lyman 2001).
We interpret this observation in terms of a model in which temporal separation between two types of DNA damage tolerance: recombination-based template switching and mutagenic translesion synthesis.
