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The AHLs production profiles of these two transformants were analyzed as described in the bioautography analysis section.

Specific growth rates and Rubisco activity levels were compared under photoheterotrophic conditions among the two transformants and the wild-type strain.

Extracellular protein concentration and the FPase activity of the other two transformants (VHb2 and VHb3) also showed a similar increase, as shown in Additional file 1: Figure S2.

Native PAGE analysis indicated that two transformants, L8 and L38, were able to secrete recombinant laccase A, and their laccase activities corresponding to ABTS oxidation reached 3.62 IU ml− 1 and 1.50 IU ml− 1 respectively.

As shown in Figure 8A, seed viability and the time course of germination were similar for untransformed control and two transformants with a sharply different rhGCase content (63.5 and 215 mg per kg of flour).

Two transformants able to grow on supplemented glucose minimal medium were revealed, one from each group of molecular masses (Fig. 4).

The two transformant strains harboring rbcTk displayed growth under photoautotrophic and photoheterotrophic conditions, both dependent on CO2 fixation.

Two transformant BSHP strains containing pHT01ribMopt ("ribM1" and "ribM2") and four transformant strains containing pHT01 (controls) were isolated and grown in shake flasks in a medium routinely used for the analysis of the performance of industrial riboflavin production strains.

Fifteen transformants were retrieved from this transformation and subjected to RT-qPCR.

Fourteen transformants stably carried the KJhyPBase expression cassette, and TR_cKJUMPS was excised from seven of these.

Twenty, sixty, and forty transformants obtained under each set of conditions were randomly selected and analyzed.