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For H&E, IHC, BrdU, and TUNEL studies, two to three sections per specimen and at least six specimens in each group were used.
BCM was evaluated stereologically in two to three sections with the observer blinded to the origin of the sections.
Two to three sections were examined from each of three spinal cord levels (cervical, thoracic and lumbar) for each mouse.
Each aorta generated two to three sections of sufficient quality for analysis, all of which were used in neointimal area analysis.
DNA was isolated from sputum and tumour biopsies (1 15 mm, two to three sections) by protease digestion followed by phenol chloroform extraction and ethanol precipitation.
To determine rostral-caudal differences quantifications were performed in each embryo on levels of the upper and lower limbs as well as on an intermediate level between the two, collecting two to three sections for each level.
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The thin film usually comes in four-foot-long sections, with two to four sections forming a large image.
Two to four sections were routinely reviewed.
Approximately 5000 cells were harvested from two to four sections for each sample.
Two to four sections from each lung of infected rats were examined.
For each explant the amount of viable chondrocytes was calculated from the values obtained from two to four sections.
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