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The second axis explained 12% of the variance: The HC populations divided loosely into two to three clusters along the second axis.
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Taking all data into account, the resulting cluster centroids for different starting configurations are stable for two to ten clusters.
Clustering the complete data set with different random starting positions leads to very small instabilities (<0.003) for the whole range from two to twelve clusters.
Of the five cluster models (comprising two to six clusters), we regarded the four-cluster model as the optimal solution.
Three types of clusters (of two to four clusters) were formed from the 204 OHTs in Minas Gerais.
In the cluster analysis we ran separate models for two to six clusters.
In general, two to five clusters were detected for the 14 strains.
ITS1 sequences were more diverse, with two to six clusters exceeding 3% of reads depending on the species.
We tested for the presence of two to six clusters using eight clustering algorithms and observed hierarchical clustering with two clusters to be the most suitable for our dataset (Supplementary Fig. S3).
The telomeres are also found at the nuclear periphery but on the opposite side of the SPB in the proximity of the nucleolus in two to four clusters (Funabiki et al, 1993; Alfredsson-Timmins et al, 2007).
To determine the most appropriate number of clusters, two to eight cluster solutions were run.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com