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This monomer contains two additional orthogonal protecting groups at its two termini, the Fmoc and the TBDMS groups.
After DNA isolation, two termini of each subclone were sequenced using the custom primers (see Supplementary Table 3 for the primer sequences) using ABI3700 capillary sequencers.
In addition, the two termini of peptide are partially out of the active pocket of ADT, thus contributing moderately to the peptide binding.
Here we propose circular permutation as a method for tethering other protein(s) at a site(s) other than the two termini.
A short segment of helix from M-HEAT extends in the opposite direction away from the N-terminus of TRD, with a straightaway distance of 35 Å between the two termini.
The two termini were located 1.2 km apart.
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The difference between the two terminuses demonstrates just what's going wrong with Mumbai.
East of town, the route comes to a junction with SH-77S (one of SH-77S's four termini).
Today, the Eastern Suburbs & Illawarra line generally operates with five termini, at Hurstville, Mortdale, Sutherland, Cronulla and Waterfall.
The interaction between the four termini accounts for close to 75% of the stabilizing force in the dimer.
PH elements are the most frequent and are recognized in 6L, 7R, 8L, 8R, 11L, 14R, 18R and 19R, whereas nine termini (namely, 2L, 6R, 12R, 13L, 13R, 15L, 15R, 16L and 20R) showed no homology to other terminal regions.
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