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Two substitution mutants, K65R and K65A were studied.
The last two substitution patterns, 6-methoxy and 5,7-dimethoxy, could be of high interest for the design of new fluorescent materials.
Gallic acid (GA) was encapsulated with native starch (NS), native inulin (NIn), acetylated starch (AS) or acetylated inulin (AIn) (two substitution degrees each) by spray-drying, and a 22 statistical factorial design was used to evaluate each system.
In order to establish a relation between the spatial distribution of known functional groups related with inhibitory activity, two substitution patterns were explored: one with substituents at N-1, C-3, C-5 positions and another at C-2, C-3 and C5 positions.
Two substitution models were employed for inferences of ancestral states among simulated sequences.
As implemented through CIPRES, RAxML has two substitution models: GTRGAMMA and GTRCAT.
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No one believed those two substitutions!
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Only with two substitutions did Pardew change the game.
Bielsa made two substitutions, and they paid off immediately.
That would be a big, big blow for Burton's hope - and two substitutions used up before extra-time.
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