MSCV-p89c-Mybex9bMUT was generated by mutating four nucleotides of MSCV-p89c-Mybex9b by two subsequent site-directed mutagenesis according to manufacturer's instructions (QuickChange II site-directed mutagenesis kit, Stratagene, Santa Clara, CA).
Milk founder Kevin Rose's (joke) offer to revert Digg back to Version 3 if we also reneged, investor Chris Sacca's colorful description of our font choices and those two subsequent "Your Site Sucks" tips that spelled "Micael Arrington" and "Site Redisign" in the subject lines exactly like that.
Optical trap experiments with native myosin II have shown that the degree of flexibility of the heads is sufficient to allow attachment to at least three subsequent binding sites on one actin filament [ 181].
Adult males disperse much shorter distances than juveniles (median dispersal distance between centres of territory sites occupied in two subsequent years of males shifting territory site: 384, 10/90% quantile = 163/1535 m, N = 62; based on males originating from the 8 km2 area; t-test, log-transformed distances: t = −9.48, DF = 259, P<0.0001).
To analyze the produced fragments, adaptors are ligated to each restriction fragment, which then serve as oligonucleotide-binding sites for two subsequent rounds of PCR.
The 47 hidden files – alluded to in a National Archive spreadsheet but kept from the public – include one about India's National Security Guard, a commando unit that led two subsequent raids on the Golden Temple, the Sikh holy site in Amritsar.
Two subsequent studies [1], [4] were less extensive with fewer sites sampled and no specimens collected for archival purposes.
In the two subsequent instances of late reactions, urticaria appeared at the injection site, and then became widespread within 1 to 2 days.
He lost two subsequent appeals.
(He sang two subsequent performances as well).
Two subsequent marriages ended in divorce.
