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A two steps experimental procedure was followed to optimize the process.
A three step experimental design was developed based on the central composite design (CCD).
In order to optimize the purification protocol of plant expressed Nef fused to a hexa-histidine tag (His 6-tag) [ 15], a two step IMAC experimental procedure was set up using both a nickel-nitrilotriacetic acid (Ni-NTA) and cobalt affinity purification columns.
This fact is used to settle down a fitting procedure using two steps (two separated experimental sets of data concerning temperature, light intensity, oxygen, water and/or 2-propanol concentrations) with, respectively, four and three parameters.
We adjusted the values of Φ in three steps using experimental data where available.
To understand the dynamic behavior of cellular systems, mathematical modeling is often necessary and comprises three steps: (1) experimental measurement of participating molecules, (2) assignment of rate laws to each reaction, and (3) parameter calibration with respect to the measurements.
This research used two main investigative steps: experimental scaled wind tunnel testing and Computational Fluid Dynamics (CFD) simulation.
Two Step.
In all cases, the anodization was performed in two steps and under the same experimental conditions for all samples.
The optimization in two steps of seven adjustable parameters through experimental designs associated to an optimal and individualized processing of atomization data allowed to reach a detection limit of 30 ng l−1 for the quantification of Al in seawater.
As described in "The proposed methodology" section, the proposed approach employs the experimental design in two steps including the screening design (Step 1.4 of "The proposed methodology" section) and the fractional factorial design (Step 1.5 of "The proposed methodology" section).
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