The study was conducted in five university clinics in Sweden and occurred in three stages (Fig. 1); the methods of the first two stages have previously been described in detail (18).
This target state is the one in which the system should be in order to start testing and can correspond to any entry point to any of the three controller stages previously mentioned.
The goal remains the same, namely to stage the AD-related neurofibrillary pathology in six stages, as previously, with emphasis this time on the plexuses formed of both pretangle and tangle material, but using paraffin sections immunostained for hyperphosphorylated tau and processed on an automated basis.
Two stages are shown: a) having previously calculated betweenness values for this network (shown in Figure2f), the link e with the highest betweenness value in the original network is identified and highlighted red, b) the remaining network after the removal of e.
Between these last two stages, the tactoids, which were previously randomly oriented in the suspension, align to have the nematic axis perpendicular to the petri dish surface.
The mutations were made using the oligonucleotides listed below (mutations in lower case and underlined) and their reverse complements using two stage PCR as previously described [17].
As previously mentioned, the video surveillance ecosystem is divided in two stages: the implementation domain, developed envisioning the system evolution, followed by the application engineering stage.
The concentration of histidine in SAA was determined in three stages, as described previously [ 27].
Patients diagnosed with STGD1 were classified into one of the four stages as described previously [Fishman et al., 1999].
The current candidate gene-based genetic association study was performed in three stages using the previously published GWAS datasets and two new independent sample series of Chinese Han population.
