To detect stress response transcripts from all RAP transcripts and unannotated transcripts, statistical analysis by G test was conducted between two stages of stress treatments for each transcript.
Comparison of patterns of transcript accumulation at two stages of fruit development showed increased levels of gene expression for 9 of the analysed genes.
Salt-induced transcripts found in common to at least two stages of salt stress conditions.
The remaining 392 (91.0%) transcripts were differentially expressed exclusively during one of the two stages of parasite infection.
In comparison with the three stages of WT anthers, transcripts have differentially degrees of reduction in three corresponding stages of mutant anthers.
Approximately 60% of the expressed transcripts were differentially expressed between at least two out of the seven stages of berry development (28% of transcripts, 4,151 Unigenes, had pronounced (≥2 fold) differences in mRNA expression) illustrating the dynamic nature of the developmental process.
Of a total of 34,932 genes that are expressed over the three stages of mutant anthers, 16,145 transcripts were shared across all the three mutant anther stages, 9,737 were stage-specific transcripts and 9,050 genes were expressed at two stages.
In addition, 14 peroxidase genes have low levels of transcripts at the three stages of stem development analyzed.
In this study, we present the first large-scale genomic survey of O. ostertagi by the analysis of expressed transcripts from three stages of the parasite: third-stage larvae, fourth-stage larvae and adult worms.
The first three stages of the analysis were familiarization (reading the transcripts multiple times), development of a set of themes defined by the human rights framework described above [ 8], or arising naturally in the course of the interviews; and indexing and summarizing the themes and sub-themes.
This analysis revealed that none of the wild-type transcript profiles from the three stages of symbiosis matched the profile of cyclops at 21 dpi.
