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K. McKeown and Nicholas P. Jewell, "Current status observation of a two stage counting process with application to simultaneous accurate and diluted assay HIV test data," The Canadian Journal of Statistics, 2011, 39, 475-487.
The outcome of this procedure produced many islet-like cell clusters, which could express insulin and glucagon as shown in figures 1 and 2. For in vitro insulin secretion assay, twenty stage 4 cell clusters were handpicked, washed with PBS 5 times to prevent insulin assay cross reaction with medium, and then cultured in 2% FBS/DMEM (5.5 mM glucose) without B27 and nicotinamide for 24 h.
First, we determined the antioxidant activity of the three stages by DPPH assay and observed a dose-dependent relation in DPPH scavenging activity of Baizhi root extracts (Fig. 2a, b).
The assay comprises a single-step, closed-tube reaction in which PCR amplification takes place in two stages.
Actually, two stages.
It involves two stages.
Implants come in two stages.
Debt reduction took place in two stages.
Actually, this happened in two stages.
We will do this in two stages.
It was built in two stages.
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