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One might speculate that the same differences in the recognition domains of the two sigma factors may also account for the discrimination of Rsd to engage specifically with one of the two specificity factors.
Subsequent analysis, including site-directed mutagenesis studies, showed that two specificity clusters exist in each of the investigated PDZomes, and that the presence of a histidine and valine residue in alpha-helix 2 (residues αB1 and αB5, respectively) determines the binding of the most promiscuous cluster.
The two specificity categories are presented in Table 2a and distributed over the three sensitivity categories.
We computed these two specificity measures by using the biochemical pathways of E. coli obtained from the KEGG database [ 39] (left panel of Figure 8).
More interestingly, the other two "specificity" amino acids participated in contacts at the 5′ end of tRNA where the sequences of the two tRNAs were identical.
Here we describe the computational pipeline used to build a dataset of over 400 alignments of homologous enzyme domains each consisting of two specificity groups with columns likely important for specificity, as well as specificity groupings, identified.
Similar(53)
Specifically, for any two specificities chosen at random, chances were 98.3percentthatatheyey overlapped by 10percentt or less.
Satisfying the two specificities by a binary combination of Flp variants, while necessary, may not be sufficient to elicit recombination.
Bispecific molecules (e.g., bispecific tandem scFv) exhibit two specificities, often against a tumor antigen as well as an immune-activation ligand such as CD3.
However, our sample has two specificities.
Overall, for any two specificities chosen at random, the probability was 98.3 percent that their repertoires' VDJ H combinations overlapped by 10percentt or less (Fig. 2b).
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