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The program DnaSP [ 89] was used to determine the position-by-position nucleotide diversity of two small alignments derived from the full-length nucleotide dataset.
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We ran the algorithms on the full alignment and on two smaller alignments created by randomly sampling 20000 and 40000 sequences from the large alignment.
The alignment was manually truncated by the removal of twenty positions at both the N-terminal and C-terminal that contained excessive gaps and two small internal regions of poor alignment, one consisting of six residues in the linker region between the N and C domains and the second consisting of 21 residues in the C-terminal domain of which over 75% were gaps in the majority of sequences.
The rps2 alignment was simple, containing only two small indels.
Multiple localizations to a single exon can occur because masking or mismatches within exons can split what should be one long matched segment into two or more smaller alignments.
Tools, two small knives.
Two small blankets.
Get two small plates.
Get two small bowls.
Collect two small boxes.
Draw two small curves.
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