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The two sets of sections are then run through machines that collate them into complete papers.
Two sets of sections were prepared with each set consisting of 7 8 sections, 100 µm apart.
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Three sets of sections at PVN level were taken and used for Avp or Oxytocin (Oxt) in situ hybridization (ISH) according to established protocols [20], [34].
Five sets of sections were manually stained applying IHC methodology.
From two such cores, we also took three sets of sections for DNA extraction to allow assessment of heterogeneity within cores in addition to the spatial heterogeneity within and between cancerous prostates (Supplementary Figure 1a).
The two sets of tissue sections (dosed animal and spotted control) were then coated with matrix as described above using the Image Prep and subsequently analyzed by imaging mass spectrometry in MS and MS/MS mode, respectively.
Two sets of sequential sections were stained with Giemsa and alizarin red.
Two sets of serial sections (at intervals of 120 μm) were mounted on slides (Superfrost PLUS), using a solution of 0.1% BSA (albumin bovine, minimum 98%) in PBS.
One set of sections was stained with Nissl (cresyl violet).
One set of sections was stained with hematoxylin and eosin using routine procedures.
The experimental facility designed for this work is of two sets of power section that can be controlled independently to drive two co-axial disks.
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