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In all other investigations, Mann Whitney comparison of the two series of values corresponding to the tumoral and parenchyma ROIs showed statistically relevant differences between the two TICs (p < 0.001).
The agreement between the bulk and individual frequency estimates shows a substantial absence of bias: the slope of regression between the two series of values was 1·0 and the intercept was 0·0, with an R of 0·93.
The two series of values have close mean values (2.96 for P e and 3.46 for the number of RGC_CAMs supporting ecdysozoa) although the difference is statistically significant (P = 0.02) by Student's t-test).
The agreement between estimates from bulk and individuals was high: the slope of regression between the two series of values was 0·99 and the intercept was 0·00, with an R of 0·93.
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We applied the five series of values reported by Atchley et al. [ 9], to determine how the sequence variability of the orthologs affects the biochemical characteristics of the encoded proteins.
Additionally, we used five series of values for amino acid properties (polarity, secondary structure formation, volume, amino acid composition and electrostatic charge) reported by Atchley et al. [ 9], to explore the relationships of physico-chemical properties of residues encoded in the orthologs with phylogeny, evolutionary rates and function.
In Figure 8, the Hough transform of an image is shown where two series of large values in the Hough plane, corresponding to the values 18 and 27, are observed.
However, there was no correlation between the two series of pO2 values in the majority of the tumours, implying that the pO2 fluctuations in different regions of a tumour in general were temporally independent.
For example, the correlation coefficient between two series of locus-matched values obtained by distinct Authors, using different microarray platforms (samples A1 and A3, respectively; see Table 1), was 0.23 in absence of any inter-sample normalization, 0.34 following standard quantile normalization for all values and 0.41 following scaled quantile method.
A paired two-tailed Student's t test was used to detect significance between two series of data and P value (P) < 0.05 was considered statistically significant.
To determine the effect of the noise intensity, we consider three series of different values.
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