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To avoid double counting the same cell on two sequential sections, only every fifth section was counted.
Two sequential sections were placed side-by-side on a charged microscope slide (ProbOn; Fisher Scientific Co., Pittsburgh, PA, USA).
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The number of positively stained cells in the entire tibia subchondral bone area was counted in three sequential sections per mouse in each group.
The specimens were processed for micro-CT analysis, histological examination, and calculated per patient in three sequential sections with OARSI scoring system as previously described.49 The number of positively stained cells in the tibia subchondral bone area was counted in three sequential sections per patient in each group.
In contrast to presenting a stepwise description of different platforms, we discuss expression profiling of mature miRNAs by qPCR in four sequential sections: (1) cDNA synthesis; (2) primer design; (3) detection of amplified products; and (4) data normalization.
The majority of the clusters were distributed through more than three sequential sections, and several strings of microspheres were distributed through the 15 sections, indicating that some clusters may be even larger than discovered.
Four sequential sections were deposited on each slide.
Three sequential sections of each case were stained for GRP78, XBP1 and HIF-1 α.
The average number of cells counted in three sequential sections gives the number of neurons in each rat.
Sets of three sequential sections were stained with Alcian blue only, Sirius red only, and both stains.
Three sequential sections per animal comprising the OVLT through the midlevel region of the AVPV (corresponding to 0.24 through -0.12 mm from Bregma) were selected for analysis using a brain atlas for reference [ 52].
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