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It consists of two consecutive identical chromatographic separations, with a modification step targeted to a subset of peptides between the two separations.
The results showed that the JR curve had three stages, with the two separations as the initial cracking point and the crack localizing point.
Selectivity of phase system is of primary concern when designing a bidimensional chromatographic system and looking for the highest degree of orthogonality between the two separations.
In both situations, it turns out that the ratio between the two separations of scales (macroscopic scale/fissure scale and fissure scale/pore scale) plays an essential role.
Based on this analysis, the initial formation configuration was described in a series of closed relative motion ellipses by separation parameters, including the direction and size of ΔV for each payload satellite, and the time interval between two separations.
The last two separations help keeping the number of necessary resources to handle 16 instructions per cycle (IPC) the same as what is needed today to handle 4 instructions per cycle.
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Two separation methods were established where selected food additives were baseline separated.
Next, we study the relationship of the two separation conditions.
Figure 2 shows the basic structures of these two separation systems.
In this method, two separation steps are conducted namely isoelectric focusing (IEF) and SDS-PAGE.
In addition, two separation columns allow two-dimensional on-column ITP ITP- and CZE ITP-coupling.
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